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A protocol to visualize cytosolic aggresome-like bodies using confocal microscopy.
Soyeon Kim1,2, Yiseul Hwang1,2, Ho Chul Kang1,2
1Department of Physiology, Ajou University School of Medicine, Worldcup-ro, Yeongtong-gu, Suwon, Gyeonggi 16499, Republic of Korea.
STAR Protocols
|August 2, 2021
Summary
This study presents a protocol for monitoring aggresome-like bodies (ALBs) formed during ubiquitin stress. The method uses immunocytochemistry to quantify cells with ALBs, aiding in the study of cellular stress responses.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Ubiquitin stress triggers cellular responses, including the formation of aggresome-like bodies (ALBs) near the cell nucleus.
- Understanding the biological characteristics of ALBs is crucial for deciphering cellular stress mechanisms.
Purpose of the Study:
- To establish a reliable protocol for monitoring and quantifying aggresome-like bodies (ALBs) induced by cellular ubiquitin stress.
- To provide a method for characterizing the phenotypes of stress-induced ALBs.
Main Methods:
- Development of an immunocytochemistry protocol to detect and quantify ALBs.
- Optimization of the protocol using readily available materials and reagents.
Main Results:
- Successful implementation of a protocol to monitor ALBs formation under ubiquitin stress.
- Quantification of cells containing ALBs is achievable with the described method.
Conclusions:
- The presented immunocytochemistry protocol is effective for studying stress-induced ALBs.
- This method facilitates the exploration of various molecules involved in ALB formation and cellular stress response.
