Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

18.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
18.3K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

483
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
483

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo.

bioRxiv : the preprint server for biology·2026
Same author

Designer indicators for two-photon recording of subthreshold voltage dynamics.

Nature methods·2026
Same author

Adaptive optical correction for in vivo two-photon fluorescence microscopy with neural fields.

Nature methods·2026
Same author

Intelligent image-activated sorting of large cells enabled by elasto-inertial focusing.

Lab on a chip·2026
Same author

<i>In vivo</i> aberration measurement and correction for ultrafast FACED two-photon fluorescence microscopy of the brain.

bioRxiv : the preprint server for biology·2026
Same author

Convergence of Cortical and Thalamic Origins of Free Behavior Modulation of Mouse Primary Visual Cortex.

bioRxiv : the preprint server for biology·2026
Same journal

iMUT-seq mapping of DSB-induced mutations with high sensitivity at single-nucleotide resolution.

Nature protocols·2026
Same journal

An assay to quantify sexual commitment and stage conversion in the human malaria parasite Plasmodium falciparum.

Nature protocols·2026
Same journal

Author Correction: Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.

Nature protocols·2026
Same journal

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026
Same journal

Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

Nature protocols·2026
Same journal

Photocatalytic low-temperature defluorination of PTFE.

Nature protocols·2026
See all related articles

Related Experiment Video

Updated: Oct 26, 2025

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
10:53

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

10.1K

High-speed laser-scanning biological microscopy using FACED.

Queenie T K Lai1, Gwinky G K Yip1, Jianglai Wu2,3

  • 1Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong, China.

Nature Protocols
|August 3, 2021
PubMed
Summary
This summary is machine-generated.

Free-space angular-chirp-enhanced delay (FACED) microscopy enables ultrafast laser scanning for biological imaging. This protocol details FACED hardware and implementation for broader applications in high-speed microscopy and flow cytometry.

More Related Videos

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
07:53

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

8.5K
Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues
08:01

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Published on: March 1, 2024

1.1K

Related Experiment Videos

Last Updated: Oct 26, 2025

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
10:53

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

10.1K
Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
07:53

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

8.5K
Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues
08:01

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Published on: March 1, 2024

1.1K

Area of Science:

  • Advanced optical microscopy
  • Biophotonics
  • High-speed imaging

Background:

  • Laser scanning microscopy offers high contrast, resolution, and sensitivity.
  • Current methods face challenges in achieving high speeds for large sample populations or dynamic processes.
  • Ultrafast scanning is crucial for high spatiotemporal resolution in biological research.

Purpose of the Study:

  • To provide a comprehensive guide for implementing free-space angular-chirp-enhanced delay (FACED) microscopy.
  • To enable the application of FACED technology to a wider range of imaging modalities.
  • To facilitate high-speed biological imaging and analysis.

Main Methods:

  • Detailed hardware design specifications for FACED systems.
  • Step-by-step optical implementation protocols for the FACED module.
  • Image acquisition and reconstruction pipeline for FACED microscopy.
  • Demonstration of multimodal FACED imaging flow cytometry and 2D two-photon fluorescence microscopy.

Main Results:

  • FACED technology achieves ultrafast line-scan rates of 1-80 MHz, surpassing mechanical limitations.
  • Successful implementation in multimodal imaging flow cytometry (bright-field, fluorescence, SHG).
  • Demonstrated kHz 2D two-photon fluorescence microscopy capabilities.
  • The protocol enables setup within 2-3 months for users with basic optics and software experience.

Conclusions:

  • FACED is a versatile, all-optical, passive, and reconfigurable laser-scanning approach.
  • This protocol empowers broader adoption of FACED for high-speed biological imaging applications.
  • FACED significantly enhances the ability to study dynamic biological processes and large cell populations.