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Updated: Oct 26, 2025

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
Published on: June 19, 2019
A role for the cortex in sleep-wake regulation
Lukas B Krone1,2, Tomoko Yamagata2,3, Cristina Blanco-Duque1,2,4
1Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
Abstract:
Cortical and subcortical circuitry are thought to play distinct roles in the generation of sleep oscillations and global state control, respectively. Here we silenced a subset of neocortical layer 5 pyramidal and archicortical dentate gyrus granule cells in male mice by ablating SNAP25. This markedly increased wakefulness and reduced rebound of electroencephalographic slow-wave activity after sleep deprivation, suggesting a role for the cortex in both vigilance state control and sleep homeostasis.
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