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HLA-B*27 typing using a triplex real time PCR in routine laboratory
Marte K Viken1, Alice L Pedersen1, Menaka Andersen1
1Department of Immunology, Oslo University Hospital and University of Oslo, Oslo, Norway.
Abstract:
We have established an in-house HLA-B*27 multiplex typing assay by using a combination of previously published, newly designed and commercial primers and probes for use with real-time PCR instruments. Hence, facilitating quick and large-scale HLA-B*27 typing.
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