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Updated: Sep 30, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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Characterisation of the Novel HLA-DQB1*04:115N Allele by Next- and Third-Generation Sequencing

Elham Harfouch1, Romain Ferru-Clement2, Isabelle Jollet2

  • 1EFS Île-de-France, Laboratoire d'Histocompatibilité et d'Immunologie Leuco-Plaquettaire, Créteil, France.

HLA
|September 29, 2026
PubMed

Keywords:
HLA‐DQB1*04:115NHLAOxford nanopore technologiesnull allelesequencing‐by‐synthesis

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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.