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Rapid colorimetric analysis of multiple microRNAs using encoded hydrogel microparticles
Ju Yeon Kim1, Seok Joon Mun1, Yoon Ho Roh1
1Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea. bong98@korea.ac.kr.
The Analyst
|August 4, 2021
Summary
This study introduces a faster colorimetric assay for microRNA (miRNA) detection using encoded hydrogel microparticles. The enhanced method significantly reduces assay time while maintaining sensitivity and accuracy for diagnosing diseases.
Area of Science:
- Biomarker Discovery
- Nanotechnology
- Analytical Chemistry
Background:
- MicroRNAs (miRNAs) are crucial biomarkers for diagnosing fatal diseases.
- Colorimetric bead-based assays offer simple, portable, and cost-effective miRNA detection.
- Encoded hydrogel microparticle assays show promise but are limited by long assay times.
Purpose of the Study:
- To develop a rapid colorimetric assay for miRNA detection using encoded hydrogel microparticles.
- To address the limitation of prolonged assay time in existing hydrogel microparticle-based methods.
- To improve the practical applicability and detection accuracy of miRNA assays.
Main Methods:
- Post-synthesis functionalization to increase enzyme loading on hydrogel microparticles.
- Elevated enzyme reaction temperature during colorimetric labeling to accelerate the reaction.
- Validation of sensitivity and multiplexing capability with different miRNA targets.
Main Results:
- Significantly reduced colorimetric reaction time achieved through increased enzyme immobilization and higher reaction temperature.
- Comparable sensitivity demonstrated for three different miRNA targets despite the shortened assay time.
- Method validated for multiplex miRNA detection with low cross-reactivity.
Conclusions:
- The developed rapid colorimetric assay overcomes the time limitations of previous hydrogel microparticle methods.
- The enhanced assay maintains diagnostic potential for various diseases through sensitive and accurate miRNA detection.
- This technique is suitable for practical, on-site multiplex miRNA analysis.
