Related Experiment Videos
Evaluation of changes in cytoplasmic pH in thrombin-stimulated human platelets
T A Davies1, J M Dunn, E R Simons
1Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118.
Analytical Biochemistry
|November 15, 1987
Abstract:
Thrombin causes a dose-dependent cytoplasmic alkalinization of normal human platelets. The pH probe 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) permits an easier and more accurate quantitation of the kinetics of this change previously measured with 9-aminoacridine and 6-carboxyfluorescein (6-CF). We report here a modification of the previously published 6-CF technique and confirm the thrombin-induced cytoplasmic pH change in the human platelet using a second fluorescein derivative, BCECF. Maximal thrombin stimulation raises the resting cytoplasmic pH of the human platelet from 7.0 to 7.25.