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A reversed-phase high-performance liquid chromatographic method for characterization of biosynthetic human growth
R M Riggin1, G K Dorulla, D J Miner
1Bioanalytical Development Department, Eli Lilly and Company, Indianapolis, Indiana 46285.
Analytical Biochemistry
|November 15, 1987
Abstract:
An isocratic reversed-phase high-performance liquid chromatographic method for the determination of human growth hormone (HGH) purity is described. This method offers superior resolution of HGH-related substances (e.g., sulfoxide and desamido derivatives) from unmodified HGH when compared to a number of alternative chromatographic and electrophoretic techniques.