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Detection of scorpion venom by optical circular dichroism method
1Magneto-Plasmonic Lab, Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran.
Scientific Reports
|August 5, 2021
Summary
This study demonstrates a sensitive method using plasmonic structures and circular dichroism (CD) to detect scorpion venom concentrations and neurotoxic effects in human blood serum. The developed sensor accurately measures venom levels, enhancing safety and lifestyle. Keywords: scorpion venom detection, neurotoxic effect, circular dichroism, biosensor.
Area of Science:
- Analytical Chemistry
- Biophysics
- Materials Science
Background:
- Developing high-sensitivity, accurate, and low-cost sensors is crucial for detecting toxic materials and venom's neurotoxic effects.
- Existing methods for detecting venom (Odontobuthus Doriae Scorpion) in human blood serum face challenges in sensitivity and cost-effectiveness.
Purpose of the Study:
- To investigate the reliability of a two-dimensional plasmonic structure combined with circular dichroism (CD) for detecting venom concentrations.
- To determine the neurotoxic effect of Odontobuthus Doriae Scorpion venom on human blood serum neurotransmitter analytes.
- To assess the sensor's sensitivity and accuracy in measuring venom and its impact.
Main Methods:
- Utilized a two-dimensional plasmonic structure as a sensor platform.
- Employed circular dichroism (CD) spectroscopy to analyze toxic samples.
- Measured venom concentrations and their neurotoxic effects on neurotransmitter analytes in human blood serum.
Main Results:
- Confirmed a direct dependency between the CD signal and the neurotoxic effect of venom concentrations.
- Demonstrated good sensitivity of the sensor, even with an achiral plasmonic structure.
- Successfully measured venom concentrations and assessed their neurotoxic impact.
Conclusions:
- The combination of a two-dimensional plasmonic structure and CD provides a reliable and sensitive method for detecting scorpion venom.
- This sensor technology can accurately determine venom concentrations and their neurotoxic effects in human blood serum.
- The findings contribute to enhanced safety and lifestyle through improved toxic material detection.
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