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Human leucocyte migration: studies with an improved skin chamber technique.
Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology
|December 1, 1977
Summary
A new skin chamber method effectively measures localized leukocyte mobilization (LLM) in humans. This technique shows reduced LLM in severe infections and leukemia, offering a valuable tool for studying cell migration.
Area of Science:
- Immunology
- Dermatology
- Cell Biology
Background:
- Localized leukocyte mobilization (LLM) is crucial for immune response.
- Existing methods for studying LLM can be complex or lack reproducibility.
Purpose of the Study:
- To develop and validate an improved skin chamber technique for studying LLM.
- To assess LLM in healthy individuals and patients with various conditions.
Main Methods:
- A suction device created uniform dermal windows on forearm skin.
- Blister formation was induced, and the blister base was covered with a chamber containing autologous serum.
- Chambers were harvested at 2, 4, and 24 hours to quantify cell migration.
Main Results:
- Healthy individuals showed a significant increase in leukocyte counts over 24 hours (median 1.9 x 10^6 at 4h, 3.8 x 10^7 at 24h).
- Subnormal LLM was observed in patients with severe bacterial infections and leukemia.
- Emigrated neutrophils exhibited functional derangements, including reduced bactericidal capacity.
Conclusions:
- The improved skin chamber technique is simple, reproducible, and valuable for LLM studies.
- This method can differentiate LLM in healthy individuals versus those with specific diseases.
- Functional analysis of emigrated leukocytes provides insights into immune cell derangement.