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Age Estimation with Cemental Annulation Using Light, Phase Contrast and Polarized Microscopy.

L Pradeep1, Ganganna Kokila2, Pillai Arun Gopinathan3

  • 1Department of Dentistry, Sapthagiri Institute of Medical Sciences and Research Centre, Bangalore, Karnataka, India.

Journal of Microscopy and Ultrastructure
|August 5, 2021
PubMed
Summary
This summary is machine-generated.

Accurate age estimation in forensic science is crucial. This study found cemental annulation using phase contrast microscopy reliable for determining age, particularly in younger and middle-aged individuals.

Keywords:
Cemental annulationsphase contrast microscopypicrosirius red stainpolarized microscopy

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Area of Science:

  • Forensic Odontology
  • Biomaterials Science

Background:

  • Age determination is vital in forensic science for identification.
  • Teeth, particularly cementum, offer reliable age estimation due to preserved annulations.
  • Microscopic analysis of cemental annulations aids in forensic age assessment.

Purpose of the Study:

  • To compare the accuracy and efficiency of age estimation using different microscopy techniques.
  • To evaluate light, polarized, and phase-contrast microscopy on ground and decalcified tooth sections.
  • To assess the utility of hematoxylin and eosin (H and E) and Picrosirius Red (PSR) stains.

Main Methods:

  • Fifty teeth were sectioned into ground and decalcified samples.
  • Ground sections were analyzed using light, polarized, and phase-contrast microscopy.
  • Decalcified sections were stained with H and E and PSR, then examined microscopically.

Main Results:

  • No significant age difference in ground sections.
  • Significant age differences observed in decalcified sections (H and E, PSR).
  • Strong positive correlation found between actual and calculated ages.

Conclusions:

  • Cemental annulation analysis with phase contrast microscopy is reliable for age estimation.
  • This method is particularly effective for young and middle-aged individuals.
  • Microscopic age estimation from tooth cementum is a valuable forensic tool.