Preoperative imaging of glioblastoma patients using hyperpolarized ¹³C pyruvate: Potential role in clinical decision...

Glioblastoma remains incurable despite treatment with surgery, radiation therapy, and cytotoxic chemotherapy, prompting the search for a metabolic pathway unique to glioblastoma cells.¹³C MR spectroscopic imaging with hyperpolarized pyruvate can demonstrate alterations in pyruvate metabolism in these tumors.

Three patients with diagnostic MRI suggestive of a glioblastoma were scanned at 3 T 1-2 days prior to tumor resection using a ¹³C/¹H dual-frequency RF coil and a ¹³C/¹H-integrated MR protocol, which consists of a series of ¹H MR sequences (T₂ FLAIR, arterial spin labeling and contrast-enhanced [CE] T₁) and ¹³C spectroscopic imaging with hyperpolarized [1-¹³C]pyruvate. Dynamic spiral chemical shift imaging was used for ¹³C data acquisition. Surgical navigation was used to correlate the locations of tissue samples submitted for histology with the changes seen on the diagnostic MR scans and the ¹³C spectroscopic images.

Each tumor was histologically confirmed to be a WHO grade IV glioblastoma with isocitrate dehydrogenase wild type. Total hyperpolarized ¹³C signals detected near the tumor mass reflected altered tissue perfusion near the tumor. For each tumor, a hyperintense [1-¹³C]lactate signal was detected both within CE and T₂-FLAIR regions on the ¹H diagnostic images (P = .008). [¹³C]bicarbonate signal was maintained or decreased in the lesion but the observation was not significant (P = .3).

Prior to surgical resection, ¹³C MR spectroscopic imaging with hyperpolarized pyruvate reveals increased lactate production in regions of histologically confirmed glioblastoma.