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Updated: Oct 25, 2025

DNA Methylation: Bisulphite Modification and Analysis

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Introduction to Single-Cell DNA Methylation Profiling Methods.

Jongseong Ahn¹, Sunghoon Heo¹, Jihyun Lee^2,3

¹Department of Chemistry, Yonsei University, Seoul 03722, Korea.

|August 6, 2021

Summary

This summary is machine-generated.

Single-cell DNA methylation profiling reveals differences between cells. This review covers methods, analysis, applications, and future directions for this epigenetic marker in research and disease.

Keywords:

DNA methylation bioinformatics single cell

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Last Updated: Oct 25, 2025

DNA Methylation: Bisulphite Modification and Analysis

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Published on: October 21, 2011

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Area of Science:

Epigenetics
Molecular Biology
Genomics

Background:

DNA methylation is a key epigenetic mechanism regulating cellular differentiation and gene expression in mammals.
It serves as a crucial marker for identifying cellular heterogeneity and has implications in various diseases.

Purpose of the Study:

To provide a comprehensive overview of single-cell DNA-methylation profiling methods.
To discuss computational data analysis approaches and limitations of current techniques.
To highlight applications and future prospects in the field.

Main Methods:

Review of experimental strategies for single-cell DNA-methylation profiling.
Exploration of computational approaches for analyzing single-cell methylation data.
Discussion of limitations and emerging alternatives in data analysis.

Main Results:

Single-cell DNA methylation analysis enables precise identification of cell-to-cell variations.
The review details various profiling techniques and their associated analytical pipelines.
Current methods have limitations, but recent advancements offer improved solutions.

Conclusions:

Single-cell DNA methylation profiling is a powerful tool for understanding cellular processes and disease mechanisms.
The review provides insights into the current landscape and future trajectory of the field.
Advancements in methodology and analysis are crucial for unlocking the full potential of single-cell epigenetics.