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Updated: Oct 25, 2025
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
Circ_CLIP2 promotes glioma progression through targeting the miR-195-5p/HMGB3 axis
Bing Xiao1, Shi-Gang Lv1, Miao-Jing Wu1
1Department of Neurosurgery, Second Affiliated Hospital of Nanchang University, No. 1, Minde Road, Nanchang, 330006, Jiangxi Province, People's Republic of China.
Background:
Circular RNA (circRNA) has been demonstrated to play key roles in regulating glioma progression. Understanding the regulatory mechanism of circRNA in glioma is vital to reveal the pathogenesis of glioma and develop novel therapeutic strategies. Therefore, our study focuses on the role and underlying mechanism of Circ_CLIP2 in glioma.
Methods:
The expression of Circ_CLIP2, miR-195-5p and HMGB3 in glioma cells and tissues were analyzed using qRT-PCR. Cell proliferation was determined with colony formation and MTT assays. Cell cycle and apoptosis were examined by flow cytometry. Western blot was conducted for analyzing HMGB3, PCNA, Bax, Bcl-2, cleaved-caspase 3, Wnt-1 and β-catenin. Dual-luciferase reporter assay was measured to investigate the interaction among Circ_CLIP2, miR-195-5p and HMGB3.
Results:
The expression of Circ_CLIP2 and HMGB3 were increased while miR-195-5p was down-regulated in glioma cells and patients. Silencing of Circ_CLIP2 inhibited cell proliferation, enhanced cell apoptosis and inhibited the Wnt/β-catenin signaling pathway. Circ_CLIP2 suppressed miR-195-5p expression by directly sponging miR-195-5p. MiR-195-5p inhibited HMGB3 expression via directly targeting HMGB3. Knockdown of miR-195-5p facilitated cell proliferation, inhibited cell apoptosis and activated Wnt/β-catenin signaling, which were reversed by silencing of HMGB3.
Conclusion:
Knockdown of Circ_CLIP2 suppresses glioma progression by targeting miR-195-5p/HMGB3 thus inhibiting Wnt/β-catenin signaling. This study may provide potential therapeutic targets against glioma.
Insights
Circular RNA Circ_CLIP2 promotes glioma by targeting miR-195-5p and HMGB3, inhibiting the Wnt/β-catenin pathway. Silencing Circ_CLIP2 offers a potential therapeutic strategy for glioma treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) are crucial in regulating glioma progression.
- Understanding circRNA mechanisms is vital for glioma pathogenesis and therapy.
- This study investigates the role of Circ_CLIP2 in glioma.
Purpose of the Study:
- To elucidate the role and mechanism of Circ_CLIP2 in glioma.
- To explore the regulatory axis of Circ_CLIP2/miR-195-5p/HMGB3.
- To assess the impact on the Wnt/β-catenin signaling pathway.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
- Cell proliferation, cycle, and apoptosis assays (MTT, colony formation, flow cytometry).
- Western blot and dual-luciferase reporter assays to confirm molecular interactions.
Main Results:
- Circ_CLIP2 and HMGB3 were upregulated, while miR-195-5p was downregulated in glioma.
- Circ_CLIP2 knockdown inhibited proliferation, induced apoptosis, and suppressed Wnt/β-catenin signaling.
- Circ_CLIP2 sponged miR-195-5p, which targeted HMGB3, impacting glioma progression.
Conclusions:
- Circ_CLIP2 knockdown suppresses glioma by regulating the miR-195-5p/HMGB3 axis and inhibiting Wnt/β-catenin signaling.
- This pathway presents potential therapeutic targets for glioma treatment.
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