[Oxidation-reduction potential of tissues of the oral mucosal wound surface under the photodynamic action]

The purpose of the study was to examine the condition of the pro-antioxidant balance of the homogenates of tissues of the oral mucosal wound surface in vivo experiment under the photodynamic action of combined two-wave radiation to eliminate its damaging effect on the structures of cell membranes.

On the mucous membrane of the cheeks of sexually mature female autocrats of Wistar rats (n=72) experimental models of wound surfaces with a diameter of 3 mm were formed. The animals were divided into groups: control (n=36) and experimental (n=36). In the experimental group, a single photodynamic exposure was carried out for the 1st postoperative day with a sequential combined light radiation at two wavelengths (660 nm and 405 nm) with a total dose of 15 J/cm². Wound management was carried out traditionally in the control group of animals. The observation periods were 3, 7 and 14 days.

Macroscopic examination of the wound surface indicated complete epithelialization of the wound on the 7th day in the experimental group of animals and on the 14th day in the control group. Significant and sharp decline in free-radical oxidation processes (Imax, S, Z index) intensity was recorded in the experimental animals at earlier periods in comparison with the initial data and control results, when analyzing the results of studies of homogenates of animal tissues. A similar pattern was observed in the evaluation of lipid peroxidation products, especially in the early days of observations. The reported results were supported by the activation of antioxidant enzymes in the experimental group compared to control data and baseline data.

Thus, a combined photodynamic action using two wavelengths with a combined dose of 15 J/cm² provides safety and low invasiveness for use on the oral mucous membrane and, may be used to stimulate regeneration of the wound surface, which requires further experimental testing.