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Updated: Oct 25, 2025
Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices
Published on: April 22, 2022
[Colonization activity of conditionally pathogenic microorganisms and features of biofilm formation on the surface of
N B Astashina1, A P Godovalov1, E P Rogozhnikova1
1Perm State Medical University named after academician E.A. Wagner of the Ministry of Healthcare of the Russian Federation, Perm, Russia.
The Purpose Of The Study:
Was to assess the microbial colonization and biofilm-forming activity of conditionally pathogenic microorganisms in vitro to samples of acrylic-free thermoplastic polymer material and to assess the possibility of its use for the manufacture of removable tooth splinting structures by injection method.
Materials And Methods:
The reference strains S. aureus, S. epidermidis, S. pyogenes, E. faecalis, E. coli, and C. albicans. Biofilm-forming activity was assessed by the level of ethanol extraction of a 0.1% aqueous solution of gentian violet by measuring on a PowerWave X microplate reader (USA).
Results:
A low degree of severity of microbial colonization and sufficient colonization resistance to the formation of a microbial biofilm of E. coli (0.374±0.056 cu opt. Pl.), S. aureus (0.272±0.039 cu. Opt. Pl.), S. epidermdis (0.299±0.028 cu opt. Pl.), S. ryogenes (0.399±0.069 cu opt. Pl.), E. faecalis (0.401±0.089 cu opt. Pl.). Moreover, C. albicans strains form a more pronounced biofilm (0.425±0.104 cu opt. Pl.) in comparison with other strains studied in the experiment.
Conclusion:
Samples from a thermoplastic polymer based on polyoxymethylene have satisfactory biomedical characteristics and can be used at the stages of dental orthopedic treatment of patients without oral candidiasis.
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