Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

432
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
432
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

6.1K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
6.1K
Sampling Methods: Sample Types01:18

Sampling Methods: Sample Types

595
Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
595
Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

533
Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
533

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From a clinical case to a general methodology to analyze prosthetic joint failure, by micro- and nano-characterization with SEM of intra-tissue wear debris.

Applied microscopy·2025
Same author

Patient-Self Inflicted Lung Injury (P-SILI): An Insight into the Pathophysiology of Lung Injury and Management.

Journal of clinical medicine·2025
Same author

Rietveld Refinement of Electron Diffraction Patterns of Nanocrystalline Materials Using MAUD: Two-Beam Dynamical Correction Implementation and Applications.

Materials (Basel, Switzerland)·2025
Same author

The NeuroML ecosystem for standardized multi-scale modeling in neuroscience.

eLife·2025
Same author

Nanoencapsulation of Anthocyanins from Red Cabbage (<i>Brassica oleracea</i> L. var. <i>Capitata f. rubra</i>) through Coacervation of Whey Protein Isolate and Apple High Methoxyl Pectin.

Antioxidants (Basel, Switzerland)·2023
Same author

A novel path towards limiting non-exhaust particulate matter emissions of a commercial friction material through the addition of metallurgical slag.

Scientific reports·2023

Related Experiment Video

Updated: Oct 25, 2025

Experimental Protocol to Investigate Particle Aerosolization of a Product Under Abrasion and Under Environmental Weathering
07:47

Experimental Protocol to Investigate Particle Aerosolization of a Product Under Abrasion and Under Environmental Weathering

Published on: September 16, 2016

7.5K

A new sample preparation protocol for SEM and TEM particulate matter analysis.

Ankur Sinha1, Gloria Ischia1, Giovanni Straffelini1

  • 1Department of Industrial Engineering, University of Trento, Via Sommarive 9, 38123 Trento, Italy.

Ultramicroscopy
|August 6, 2021
PubMed
Summary
This summary is machine-generated.

A novel method simplifies preparing particulate matter for electron microscopy. This technique efficiently extracts particles, preserving crucial information for scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis.

Keywords:
Extraction replicasParticulate matterScanning electron microscopyTransmission electron microscopy

More Related Videos

Scanning Electron Microscopy SEM Protocols for Problematic Plant, Oomycete, and Fungal Samples
10:57

Scanning Electron Microscopy SEM Protocols for Problematic Plant, Oomycete, and Fungal Samples

Published on: February 3, 2017

29.5K
Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments
08:31

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

Published on: June 27, 2022

1.9K

Related Experiment Videos

Last Updated: Oct 25, 2025

Experimental Protocol to Investigate Particle Aerosolization of a Product Under Abrasion and Under Environmental Weathering
07:47

Experimental Protocol to Investigate Particle Aerosolization of a Product Under Abrasion and Under Environmental Weathering

Published on: September 16, 2016

7.5K
Scanning Electron Microscopy SEM Protocols for Problematic Plant, Oomycete, and Fungal Samples
10:57

Scanning Electron Microscopy SEM Protocols for Problematic Plant, Oomycete, and Fungal Samples

Published on: February 3, 2017

29.5K
Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments
08:31

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

Published on: June 27, 2022

1.9K

Area of Science:

  • Environmental Science
  • Materials Science
  • Analytical Chemistry

Background:

  • Particulate matter analysis requires specialized sample preparation for electron microscopy.
  • Existing methods can be complex and may alter particle morphology.
  • Environmental sampling systems collect diverse particulate matter, necessitating versatile preparation techniques.

Purpose of the Study:

  • To develop a new, efficient methodology for preparing particulate matter specimens for electron microscopy (SEM and TEM).
  • To enable direct observation of extracted particles with minimal interference from collection substrates.
  • To preserve topological and structural information of particulate matter during sample preparation.

Main Methods:

  • Developed a protocol for extracting particulate matter from various harvesting substrates.
  • Utilized scanning electron microscopy (SEM), including low-vacuum mode, for direct particle observation.
  • Employed transmission electron microscopy (TEM) by embedding extracted particles in an electron-transparent carbon film after substrate dissolution.

Main Results:

  • Successfully extracted and analyzed particulate matter from brake pad and disc tests using the new methodology.
  • Demonstrated the preservation of topological information in SEM specimens.
  • Achieved electron-transparent samples suitable for TEM analysis.

Conclusions:

  • The new methodology offers a versatile and effective approach for preparing particulate matter for electron microscopy.
  • Complete particle extraction minimizes substrate interference, enhancing analytical accuracy.
  • The technique is adaptable to various particulate matter types and collection substrates.