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Related Experiment Video

Updated: Oct 25, 2025

Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research

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Published on: February 16, 2024

Transient absorption spectroscopy to explore cellular pathways to photobiomodulation.

Sean P O'Connor¹, Samantha M Powell², John M Rickman³

¹Department of Physics and Astronomy, Texas A&M University, College Station, TX 77843, United States of America.

Journal of Photochemistry and Photobiology. B, Biology

|August 7, 2021

Summary

Photobiomodulation (PBM) using continuous wave (CW) laser diodes did not alter transient absorption spectroscopy (TAS) data in cytochrome c. The novel CW-TAS method shows PBM light doses tested do not affect this protein

Keywords:

Cytochrome c Low-level laser Low-level light Photobiomodulation Transient absorption spectroscopy

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Area of Science:

Biophysics
Photochemistry
Spectroscopy

Background:

Photobiomodulation (PBM) utilizes red to near-infrared light to stimulate biological effects.
Current techniques often focus on downstream effects, not the initial electronic transitions.
Studying PBM's direct impact on proteins requires advanced spectroscopic methods.

Purpose of the Study:

To introduce a novel method, CW-transient absorption spectroscopy (CW-TAS), for studying PBM effects on individual proteins.
To test the CW-TAS system's efficacy using reduced cytochrome c (Cyt c) as a model.
To investigate if low-irradiance CW light alters electronic transitions in Cyt c.

Main Methods:

Combined femtosecond transient absorption spectroscopy (TAS) with continuous wave (CW) laser diodes.
Excited Cyt c with a 532-nm laser and probed with a 350–600 nm supercontinuum.
Exposed Cyt c to CW laser diodes (450 nm, 635 nm, 808 nm) at specific irradiances.

Main Results:

CW irradiation at tested doses did not significantly alter Cyt c's TAS data compared to controls.
No new electronic transient signals were observed beyond background noise.
Kinetic analysis confirmed existing transients in Cyt c remained unchanged within experimental uncertainty.

Conclusions:

The tested doses of CW light do not affect the transient absorption properties of cytochrome c.
The developed CW-TAS technique is a promising tool for future PBM research on other proteins and complexes.
Further studies can explore PBM effects on other proteins, like Complex IV, using CW-TAS.