Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

656
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
656
Electrophoresis: Overview01:20

Electrophoresis: Overview

2.9K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
2.9K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

502
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
502

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patterns of unfinished care among nursing assistants in long-term care homes in China: a latent class analysis.

BMJ open·2026
Same author

Dual carbon source driven metabolic coupling shapes microalgal-bacterial granular sludge stability.

Environmental research·2026
Same author

First water-to-ligand substitution dictates Co<sup>2+</sup>/Ni<sup>2+</sup> extraction selectivity.

Physical chemistry chemical physics : PCCP·2026
Same author

Correction: A novel nomogram for predicting early postoperative cerebral desaturation events after congenital heart surgery.

Frontiers in surgery·2026
Same author

Ultrahigh Resolution and Quantitative Analysis of Native Protein Assembly Intermediates by Multistack Conformation-Specific Electrophoresis.

Analytical chemistry·2026
Same author

Structure-Guided Development of Genotype-Specific Recombinant Antibodies for Rapid Norovirus Detection via Dual-Mode Immunoassays.

Analytical chemistry·2026

Related Experiment Video

Updated: Oct 25, 2025

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Published on: April 29, 2020

6.4K

Cr speciation analysis based on electrokinetic sample pretreatment with a paper based analytical device.

Hui-Min Zhai1, Bin Ji2, Shan-Shan Tian1

  • 1Research Center for Analytical Sciences, Chemistry Department, College of Sciences, Northeastern University, Shenyang, 110819, China.

Talanta
|August 8, 2021
PubMed
Summary

A novel paper-based analytical device (PAD) enables rapid chromium (Cr) speciation analysis. This method efficiently separates and recovers Cr (VI) and Cr (III) without complex pretreatment, offering a cost-effective solution for environmental monitoring.

Keywords:
CrElectrokinetic stackingPADsSample pretreatmentSpeciation analysis

More Related Videos

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Published on: April 3, 2015

11.6K
Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
10:44

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies

Published on: July 1, 2016

11.6K

Related Experiment Videos

Last Updated: Oct 25, 2025

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Published on: April 29, 2020

6.4K
Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Published on: April 3, 2015

11.6K
Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
10:44

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies

Published on: July 1, 2016

11.6K

Area of Science:

  • Analytical Chemistry
  • Environmental Science

Background:

  • Chromium speciation is crucial for environmental risk assessment.
  • Traditional methods for chromium speciation often involve complex and time-consuming pretreatment steps.
  • Distinguishing between Cr (VI) and Cr (III) is essential due to their differing toxicities.

Purpose of the Study:

  • To develop a novel, simplified method for chromium speciation analysis.
  • To utilize a paper-based analytical device (PAD) for efficient separation and recovery of Cr (VI) and Cr (III).
  • To demonstrate the applicability of the method for real-world samples, such as electroplating wastewater.

Main Methods:

  • Development and optimization of electrokinetic separation and stacking on a PAD.
  • Characterization of separation and recovery using UV-Vis spectrophotometry.
  • Validation of the method using atomic spectroscopy techniques (GF-AAS and ICP-OES).

Main Results:

  • Achieved effective separation and recovery of Cr (VI) and Cr (III) without subtraction treatment.
  • Obtained limits of quantification (LOQ) of 19.0 μg L⁻¹ for Cr (VI) and 28.7 μg L⁻¹ for Cr (III).
  • Demonstrated high recoveries for both species (88-108% for Cr (VI) and 90-110% for Cr (III)).
  • Successfully applied the method for direct speciation analysis in electroplating wastewater.

Conclusions:

  • The PAD-based method offers a fast, inexpensive, and user-friendly approach for Cr speciation.
  • This technique complements atomic spectroscopy by providing speciation discrimination capabilities.
  • The method holds significant potential for routine environmental monitoring and speciation analysis of chromium.