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Selective and sensitive UHPLC-ESI-Orbitrap MS method to quantify protein oxidation markers.

Mahesha M Poojary1, Brijesh K Tiwari2, Marianne N Lund3

  • 1Department of Food Science, University of Copenhagen, Rolighedsvej 26, 1958, Frederiksberg C, Denmark.

Talanta
|August 8, 2021
PubMed
Summary

A new method quantifies 18 aromatic amino acid oxidation products in food and biological samples. This technique accurately measures these compounds, crucial for understanding oxidative stress and food quality.

Keywords:
Analytical method validationProtein hydrolysisProtein nitrationProtein oxidationReactive oxygen speciesTryptophan metabolism

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Food Science

Background:

  • Oxidative stress impacts biological systems and food quality.
  • Quantifying aromatic amino acid oxidation products is vital for health and safety assessments.
  • Existing methods lack the specificity and scope for comprehensive analysis.

Purpose of the Study:

  • To develop and validate a targeted ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method.
  • To simultaneously quantify 18 free and protein-bound aromatic amino acid oxidation products.
  • To resolve critical isomeric pairs and diastereomers without derivatization.

Main Methods:

  • Developed a targeted UHPLC-MS/MS isotopic dilution method.
  • Employed stable isotope-labeled internal standards for accurate quantification.
  • Validated the method for reproducibility, accuracy, precision, and limits of quantification.
  • Tested acid and enzymatic hydrolysis for protein-bound analytes.
  • Applied the method to diverse matrices including serum, tissue, milk, infant formula, and meats.

Main Results:

  • Achieved chromatographic resolution of all target analytes, including isomeric pairs.
  • Demonstrated method validation with excellent reproducibility (0.1-0.6% CV) and accuracy (±1-20%).
  • Quantified analytes in various food and biological samples.
  • Identified key oxidation products like o-Tyr and dioxyindolylalanine diastereomers using Fenton's reagent.
  • Observed preferential formation of o-Tyr over m-Tyr from phenylalanine.

Conclusions:

  • The developed UHPLC-MS/MS method is highly selective and accurate for quantifying aromatic amino acid oxidation products.
  • The method is robust, validated, and applicable to diverse complex matrices.
  • It provides valuable insights into oxidative processes in biological and food systems.
  • Fenton chemistry predominantly generates o-Tyr and dioxyindolylalanine diastereomers from phenylalanine and tyrosine residues.