Related Experiment Video
Updated: Aug 10, 2026
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
Published on: January 22, 2015
Chitosan nanoparticles fabricated through host-guest interaction for enhancing the immunostimulatory effect of CpG
Zhenggang Wang1, Sylvia van Drunen Littel-van den Hurk2, Lingyun Chen3
1School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha, Hunan, China; Department of Agricultural, Food and Nutritional Science, University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3, Canada.
Abstract:
CpG oligodeoxynucleotides (CpG ODNs) which can induce innate immune responses and promote adaptive immune responses, are powerful tools in defeating diseases. Here, a novel chitosan nanoparticle (CS-NPs) based on host-guest interaction has been designed for encapsulation and delivery of CpG ODNs for the first time. The CS-NPs exhibited high encapsulation efficiency (98.3%) of CpG ODNs and remained stable in storage under room temperature for at least 7 days. CS-NPs can also prevent CpG ODN diffusion at pH 7. The results of confocal laser scanning microscope images and flow cytometry show that CS-NPs can also be efficiently delivered into living cells. Furthermore, CpG@CS-NPs can increase the immunostimulatory activity of CpG ODNs. Raw 264.7 cells treated with CpG@CS-NPs demonstrated upregulation of both TNF-α and IL-6 cytokines by 13% and 40%, respectively. The newly developed CpG@CS-NPs were thus identified as an efficient system to deliver CpG-ODNs to treat various diseases.
