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Updated: Oct 25, 2025

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
Published on: January 22, 2015
New insights into physicochemical aspects involved in the formation of polyelectrolyte complexes based on chitosan
Suzana Gonçalves Carvalho1, Aline Martins Dos Santos1, Amanda Letícia Polli Silvestre1
1Department of Drugs and Pharmaceutics, School of Pharmaceutical Sciences, São Paulo State University (UNESP), 14800-903 Araraquara, SP, Brazil.
Abstract:
Polyelectrolyte complexation is a technique based on interactions between polyelectrolytes of opposite charges driven by supramolecular interactions. Although many studies address the formation of polyelectrolyte complexes (PECs), few explore strategies and tools to select the best working conditions and are often based on empirical choices. This study evaluates the influence of pH, molecular weight, and polymeric proportion on the formation of PECs based on chitosan:dextran sulfate. In addition, it assesses the approaches that study the influence of pH on the zeta potential of polymeric dispersions as a tool in the design of PECs. Results showed that nanoparticles with an excess of polycation formed aggregates, while an excess of dextran sulfate reduced the size of the particles. The graph of zeta potential as a function of pH proved to be a promising tool in the choice of polymers and a better pH condition in the development of PECs.
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