Preparation, Characterization, and Keratinocyte Cell Viability of a β‑Cyclodextrin-Myrcene Complex Intended for Skin...

Area of Science:

• Cosmetic Science
• Materials Science
• Biomedical Science

Background:

• Myrcene possesses biomedical potential but suffers from poor environmental stability and low water solubility.
• Inclusion complex formation offers a strategy to overcome these limitations and enhance myrcene's cosmetic applications.
• Beta-cyclodextrin (β-CD) is a suitable host molecule for forming inclusion complexes.

Purpose of the Study:

• To investigate the formation and physicochemical properties of a β-cyclodextrin:myrcene inclusion complex.
• To optimize the complex formation using experimental design.
• To evaluate the cytocompatibility of the inclusion complex in HaCaT keratinocytes for potential skin formulations.

Main Methods:

• Inclusion complexes were prepared via coprecipitation and optimized using response surface methodology.
• Characterization involved FTIR spectroscopy, thermal analysis (TGA, DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).
• Cytocompatibility was assessed using the MTT assay in HaCaT cells.

Main Results:

• The β-CD:myrcene complex achieved a 75.88% inclusion efficacy, confirmed by FTIR, thermal, and XRD analyses indicating successful host-guest interactions and altered crystallinity.
• SEM revealed the formation of cubic/rhomboid structures for the complex.
• While free myrcene showed good cell viability, the inclusion complex reduced viability at higher concentrations, potentially due to solubility effects.

Conclusions:

• β-CD effectively entrapped myrcene via coprecipitation, validated by solid-state and thermal analyses.
• The inclusion complex formation led to reduced cell viability compared to controls, suggesting solubility-related impacts.
• Further investigation using novel methods is needed to assess the in vitro compatibility and safety of such complexes for skin applications.