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Long-non-coding RNA RUSC1-AS1 accelerates osteosarcoma development by miR-101-3p-mediated Notch1 signalling pathway
Rui Jiang1, Ziyan Zhang2, Zhiwei Zhong3
1Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, China.
Journal of Bone Oncology
|August 9, 2021
Summary
Long non-coding RNA RUSC1-AS1 promotes osteosarcoma (OS) progression by upregulating Notch1 via the miR-101-3p pathway. Inhibiting RUSC1-AS1 may offer a therapeutic strategy for OS treatment.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Long non-coding RNA (lncRNA) RUSC1-AS1 is implicated in various cancers.
- Its specific role in osteosarcoma (OS) progression requires elucidation.
Purpose of the Study:
- To investigate the function of lncRNA RUSC1-AS1 in osteosarcoma (OS).
- To explore the underlying molecular mechanisms involving miR-101-3p and the Notch1-Ras-ERK pathway.
Main Methods:
- Quantitative Real-time PCR (qRT-PCR) and Western blot analysis were used to assess gene and protein expression.
- In vitro and in vivo gain- and loss-of-function assays were performed.
- RNA immunoprecipitation (RIP) and dual luciferase reporter gene assays confirmed molecular interactions.
Main Results:
- RUSC1-AS1 and Notch1 were significantly upregulated in OS tissues and cells.
- RUSC1-AS1 inhibition suppressed OS cell proliferation, EMT, migration, invasion, and metastasis, while promoting apoptosis.
- RUSC1-AS1 acts as a competing endogenous RNA (ceRNA) by sponging miR-101-3p, leading to increased Notch1 expression.
Conclusions:
- RUSC1-AS1 functions as an oncogenic lncRNA in osteosarcoma.
- The miR-101-3p-Notch1-Ras-ERK pathway is crucial for RUSC1-AS1-mediated OS progression.
- RUSC1-AS1 represents a potential therapeutic target for osteosarcoma.
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