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No Tumor Suppressor Role for LKB1 in Prostate Cancer.
Hikmet Koseoglu1, Asuman Celebi1, Gunay Galamiyeva1
1Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey.
DNA and Cell Biology
|August 9, 2021
Summary
The LKB1 gene shows increased expression in prostate cancer (PCa) but decreased expression in benign prostatic hyperplasia (BPH). LKB1 mutations were found in both conditions, suggesting a role in benign transformations rather than PCa development.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The LKB1 gene is implicated in cancer prognosis and frequently mutated across various cancer types.
- Understanding LKB1's role in prostate diseases is crucial for elucidating pathogenesis.
Purpose of the Study:
- To investigate the expression and mutation profile of the LKB1 gene in benign prostatic hyperplasia (BPH) and prostate cancer (PCa).
- To determine the potential association of LKB1 with the pathogenesis of prostate diseases.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) was used to analyze LKB1 gene expression.
- DNA sequencing was employed to identify mutations in the LKB1 gene.
- Analysis included 50 BPH tissues and 57 PCa tissues with matched normal controls.
Main Results:
- LKB1 expression was significantly elevated in 60.7% of PCa tissues compared to non-cancerous samples (p ≤ 0.001).
- LKB1 expression was lower in BPH tissues compared to normal tissues (p = 0.920).
- Four coding sequence alterations (3 silent, 1 missense) and intronic mutations were identified in BPH and PCa tissues.
Conclusions:
- The LKB1 gene's expression and mutation patterns suggest a potential association with benign transformations in prostate pathogenesis.
- Further research is required to fully understand the specific mechanisms of LKB1 in prostate cancer development.
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