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Related Concept Videos

Patch Clamp01:18

Patch Clamp

5.9K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
5.9K

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Related Experiment Video

Updated: Oct 25, 2025

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
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[A method of marking neurons on brain slices using patch clamp technique].

Jie Gao1,2, Yan Yun Huang1,2, Yu Tong Zhang1,2

  • 1Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan 030001, China.

Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology
|August 10, 2021
PubMed
Summary

This study presents a new method for marking neurons after patch clamp recordings. The technique allows clear visualization of neuron morphology, aiding neuroscience research.

Keywords:
brain slicesfluorescence tagsneuronal morphologywhole-cell patch clamp

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Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
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Area of Science:

  • Neuroscience
  • Cell Biology

Background:

  • Patch clamp technique is crucial for electrophysiological studies.
  • Visualizing recorded neurons post-experiment is essential for correlating function and morphology.

Purpose of the Study:

  • To develop and validate a method for marking neurons using patch clamp.
  • To enable morphological analysis of electrophysiologically characterized neurons.

Main Methods:

  • Whole-cell patch clamp recording on vibratome-cut brain slices.
  • Intracellular perfusion with Neurobiotin Tracer via microelectrode.
  • Post-recording fixation, permeabilization, and Streptavidin-Texas Red staining.
  • Fluorescence microscopy for visualization.

Main Results:

  • Successful patch clamp recordings with stable voltage and current measurements.
  • Demonstrated ability to induce action potentials in current clamp mode.
  • Clear visualization of neuron morphology, including cell body and protrusions, post-staining.

Conclusions:

  • The described method effectively marks neurons for morphological analysis after patch clamp.
  • This technique is user-friendly, providing intuitive and clear imaging.
  • It enhances the understanding of neuronal structure-function relationships.