Related Experiment Video
Updated: Oct 25, 2025

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
Published on: November 10, 2015
HRM-PCR in medical diagnostic
Damian Nikodem1, Tomasz Cłapa1, Dorota Narożna1
1Katedra Biochemii i Biotechnologii, Uniwersytet Przyrodniczy w Poznaniu. tomasz.clapa@up.poznan.pl.
Abstract:
High resolution melting (HRM) is a method based on the identification of differences in the denaturation of PCR reaction products in the presence of fluorescent dyes. It allows for a detailed analysis of the genetic profiles. In addition this analysis is low-cost, single-step, closed-tube and has high sensitivity. HRM found applications in diagnostics, laboratory and clinical researches. This article is a literature review of the applications of HRM analysis in medicine.
More Related Videos
06:30Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
Published on: February 5, 2014
07:26High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
Published on: July 18, 2017