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MiR-7-5p inhibits thyroid cell proliferation by targeting the EGFR/MAPK and IRS2/PI3K signaling pathways

Alice Augenlicht1, Manuel Saiselet1, Myriam Decaussin-Petrucci2

  • 1Institute of Interdisciplinary Research, Université libre de Bruxelles, Brussels, Belgium.

Oncotarget
|August 12, 2021
PubMed

Insights

MicroRNA-7-5p acts as a tumor suppressor in thyroid papillary carcinoma (PTC). Its reduced expression promotes cancer cell proliferation by affecting MAPK and PI3K pathways, suggesting miR-7-5p as a potential therapeutic agent.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Aberrant microRNA (miRNA) expression is linked to tumor development.
  • MiR-7-5p is a downregulated miRNA in thyroid papillary carcinoma (PTC).

Purpose of the Study:

  • To investigate the functional role of miR-7-5p in thyroid tumorigenesis.
  • To identify pathways modulated by miR-7-5p.
  • To explore miR-7-5p as a potential therapeutic strategy.

Main Methods:

  • Transfection of TPC1 and HT-ori3 cells with miR-7-5p.
  • Global transcriptome analysis to identify modulated pathways.
  • Validation of target genes (EGFR, IRS2) in human PTC samples.

Main Results:

  • Overexpression of miR-7-5p reduced proliferation in thyroid cancer cell lines.
  • MiR-7-5p modulates the MAPK and PI3K signaling pathways.
  • EGFR and IRS2 were confirmed as targets, with inverse correlation to miR-7-5p expression in human PTC.

Conclusions:

  • MiR-7-5p exhibits tumor-suppressor activity in PTC.
  • Downregulation of miR-7-5p contributes to thyroid cancer progression.
  • Restoring miR-7-5p levels could be a novel therapeutic approach for PTC.
Keywords:
IRS2MAPKPI3KmiRNApapillary thyroid carcinoma

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