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Updated: Oct 24, 2025
An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
Published on: July 31, 2019
Probiotic biomarkers and models upside down: From humans to animals
Vladimir I Trukhachev1, Victor K Chmykhalo2, Anna A Belanova2
1Center for Agrobiotechnology, Don State Technical University, Gagarin Square 1, Rostov-on-Don, 344000, Russia; Russian State Agrarian University, Moscow Timiryazev Agricultural Academy, 49 Timiryazevskaya st., 49, Moscow, 127550, Russia.
Human-derived in vitro models offer a cost-effective and efficient approach for testing multiple probiotic properties simultaneously. This method enhances screening throughput and informativeness in animal farming applications.
Area of Science:
- Microbiology
- Animal Science
- Biotechnology
Background:
- Probiotic development for animal farming requires extensive testing of properties like adhesion, toxicity, and immune effects.
- Current methods often use separate models for each property, leading to high costs, low throughput, and limited informativeness.
- The 'one model - one test - one property' principle is inefficient for comprehensive probiotic screening.
Purpose of the Study:
- To review the application of human-derived in vitro models for assessing probiotics in animal farming.
- To analyze the concordance between human in vitro models and host-derived in vivo data.
- To highlight the benefits of using human cell-based systems for efficient probiotic screening.
Main Methods:
- Review of existing literature on human-derived in vitro models used in animal probiotic research.
- Analysis of studies comparing results from human in vitro models with in vivo animal data.
- Evaluation of the precision, informativeness, cost-effectiveness, and labor effort of these models.
Main Results:
- Human-derived in vitro systems can test multiple probiotic properties concurrently.
- These models offer reasonable precision and high informativeness compared to traditional methods.
- The approach demonstrates potential for reduced expenses and labor in probiotic screening.
Conclusions:
- Human-derived in vitro models present a viable and advantageous alternative for probiotic development in animal farming.
- These systems improve screening efficiency, reduce costs, and provide comprehensive data.
- Further research and validation can optimize the use of human cell-based assays for animal applications.
