Micro-and-nanometer topological gradient of block copolymer fibrous scaffolds towards region-specific cell regulation

Regulating cell behavior and function by surface topography has drawn significant attention in tissue engineering. Herein, a gradient fibrous scaffold comprising anisotropic aligned fibers and isotropic annealed fibers was developed to provide a controllable direction of cell migration, adhesion, and spreading. The electrospun aligned fibers were engraved to create surface gradients with micro-and-nanometer roughness through block copolymer (BCP) self-assembly induced by selective solvent vapor annealing (SVA). The distinct manipulation of cell behavior by annealed fibrous scaffolds with tailored self-assembled nanostructure and welded fibrous microstructure has been illustrated by in situ/ex situ small angle X-ray scattering (SAXS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and in vitro cell culture. Further insights into the effect of integrated gradient fibrous scaffold were gained at the level of protein expression. From the perspective of gradient topology, this region-specific scaffold based on BCP fibers shows the prospect of guiding cell migration, adhesion and spreading and provides a generic method for designing biomaterials for tissue-engineering.