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Amniotic immunoreactive trypsin in pregnancies with normal and pathological outcomes
D Laroche1, M Herlicoviez, C Pasquet
1Service de Biophysique-Radioimmunologie, Centre Hospitalier Régional Universitaire, Caen, France.
Acta Obstetricia Et Gynecologica Scandinavica
|January 1, 1987
Summary
Immunoreactive trypsin (IRT) in amniotic fluid shows potential for cystic fibrosis (CF) prenatal diagnosis. IRT levels vary with gestational age and may be altered in conditions like meconium ileus and intrauterine growth retardation.
Area of Science:
- Biochemistry
- Prenatal Diagnostics
- Genetics
Background:
- Cystic Fibrosis (CF) diagnosis relies on various methods.
- Amniotic fluid analysis offers a potential avenue for prenatal screening.
- Immunoreactive trypsin (IRT) is a biomarker under investigation.
Purpose of the Study:
- To establish normal ranges for amniotic fluid IRT.
- To evaluate IRT as a complementary test for prenatal CF diagnosis.
- To investigate IRT levels in pregnancies with meconium ileus and intrauterine growth retardation.
Main Methods:
- Collected and analyzed 219 normal amniotic fluid samples.
- Established IRT reference ranges from 14 to 40 gestational weeks.
- Conducted retrospective and prospective studies on presumed CF and affected pregnancies.
Main Results:
- IRT levels increase from 14-19 weeks, stabilize until 25 weeks, then decrease.
- 3 out of 4 presumed CF fluids showed decreased IRT.
- Highly elevated IRT observed in meconium ileus cases (530x and 18x normal).
- Mildly elevated IRT noted in 4/5 cases of severe intrauterine growth retardation.
Conclusions:
- Amniotic fluid IRT shows variable patterns in normal pregnancies.
- IRT may serve as a complementary marker in prenatal CF diagnosis.
- Elevated IRT is associated with meconium ileus and intrauterine growth retardation.