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Updated: Oct 24, 2025

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
Published on: July 17, 2018
Direct Visualization of the Multicopy Chromosomes in Cyanobacterium Synechococcus elongatus PCC 7942
Ryudo Ohbayashi1, Hirofumi Yoshikawa2, Satoru Watanabe2
1Department of Cell Genetics, National Institute of Genetics, Shizuoka, Japan.
Abstract:
Cyanobacteria are prokaryotic organisms that carry out oxygenic photosynthesis. The fresh water cyanobacterium Synechococcus elongatus PCC 7942 is a model organism for the study of photosynthesis and gene regulation, and for biotechnological applications. Besides several freshwater cyanobacteria, S. elongatus 7942 also contains multiple chromosomal copies per cell at all stages of its cell cycle. Here, we describe a method for the direct visualization of multicopy chromosomes in S. elongatus 7942 by fluorescence in situ hybridization (FISH).
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