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Updated: Oct 23, 2025
Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Published on: June 16, 2021
Prediction of oocyte quality using mRNA transcripts screened by RNA sequencing of human granulosa cells
Chengjun Liu1, Ketong Su2, Lu Chen3
1College of Animal Sciences, Jilin University, Changchun 130062, China; Beijing Dairy Cattle Center, Beijing 100192, China; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100095, China.
Research Question:
Can RNA transcripts of granulosa cells be used to assess oocyte quality? The possibility of predicting the developmental competence of oocytes by RNA sequencing analysis of granulosa cells was evaluated.
Design:
Granulosa cell samples were collected from 29 women undergoing assisted reproductive technology treatment and divided into two groups: 14 samples from the high blastocyst rate group and 15 from the low blastocyst rate group. Ten samples from each group were selected for RNA sequencing.
Results:
A total of 129 differentially expressed genes associated with high developmental competence of oocytes were identified. COL1A2, renin and COL1A1 were selected and further examined by quantitative real-time polymerase chain reaction (qRT-PCR). Expression levels of COL1A2 and renin by qRT-PCR were consistent with the results of RNA sequencing.
Conclusion:
RNA sequencing data could provide novel marker genes for the non-invasive evaluation of oocyte quality and embryo developmental competence.
