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Updated: Aug 4, 2026
Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
Published on: August 31, 2015
Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by
Yumei Li1, Zhengmao Ye2, Peng Lu3
1School of Bioscience and Biotechnology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan, 250022, China. mls_liym@ujn.edu.cn.
Abstract:
A soluble melanin pigment produced by Streptomyces sp. ZL-24 was purified and named StrSM. The elemental analysis of StrSM showed it consists of carbon, hydrogen, and oxygen. The spectrum analysis, including ultraviolet-visible absorption spectrum, Fourier-transform infrared spectrum, and pyrolysis-gas chromatography-mass spectrometry, indicated that StrSM might be pyomelanin. High performance liquid chromatography and liquid chromatography-mass spectra analysis of intermediate metabolite showed the presence of homogentisic acid (HGA). Moreover, the enzyme 4-hydroxyphenylpyruvate dioxygenase, involved in HGA biosynthesis, showed high activity during melanin production. Subsequently, a tyrosinase gene (melC2) and hydroxyphenylpyruvate dioxygenase gene double mutant demonstrated StrSM is pyomelanin. In vitro bioactivity assay showed that StrSM had excellent protective capability against SH-SY5Y cell oxidative injury. To our knowledge, the results firstly provide comprehensive data on Streptomyces pyomelanin identification and a promising candidate compound to treat oxidative injury of neurocytes.
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