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New dot-blot method for evaluating the effect of inactivators on mite and Japanese cedar pollen allergens
Megumi Yoshida1,2, Keijiro Mizukami1, Keigo Kurata2
1Department of Veterinary Microbiology I, School of Veterinary Medicine, Azabu University, Sagamihara, Japan.
Bioscience, Biotechnology, and Biochemistry
|August 19, 2021
Abstract:
As a method of evaluating the effect of inactivators on allergens while suppressing the effect of inactivator on the assay, we developed new dot-blot method that combines immunostaining and protein detection methods. This method is useful for evaluating whether the inactivator can inactivate allergens rather than removing them from the assay.
