Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Imprinting

Imprinting

Behavioral imprinting is observed in some newborn animals and occurs when they develop strong and specific attachments to another animal (usually a parent) following brief, early-life exposures. Offspring imprint onto parents within a brief period after birth or hatching; this time window is called the critical period. Once imprinting occurs, the bond established between the parents and their offspring is usually long-lasting.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Guanine-Rich DNA Aptamers for Selective Binding to Agarose Hydrogels.

Bioconjugate chemistry·2026

Same author

Capture-SELEX-Derived Low-Nanomolar-Affinity Aptamers for Doxorubicin and Inhibition of Cellular Uptake.

ACS chemical biology·2026

Same author

Enzymes, DNAzymes and nanozymes for environmental remediation.

Nanoscale·2026

Same author

Combining G-Quadruplex and Non-Quadruplex Aptamers with Distinct Thermodynamic Driving Forces for Highly Selective Pb<sup>2+</sup> Detection.

ACS sensors·2026

Same author

Capture-SELEX derived aptamers for light-up fluorescent detection of carbendazim.

Talanta·2026

Same author

Affinity sensors for L-lactate and lactate dehydrogenase.

Analytical methods : advancing methods and applications·2026

Search research articles

Related Experiment Video

Updated: Oct 23, 2025

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting

Published on: January 23, 2013

Preparing Selective Nanozymes by Molecular Imprinting.

Yuqing Li¹, Xiaohan Zhang¹, Juewen Liu²

¹Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|August 19, 2021

Summary

Researchers enhanced nanozyme selectivity using molecularly imprinted polymers (MIPs). MIP-coated iron oxide nanozymes show improved specificity for analytical and biomedical applications.

Keywords:

Catalysts Iron oxide Molecularly imprinted polymers Nanogels Nanozymes

More Related Videos

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

Related Experiment Videos

Last Updated: Oct 23, 2025

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting

Published on: January 23, 2013

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

Area of Science:

Nanomaterials Science
Biochemistry
Polymer Chemistry

Background:

Nanozymes, such as Fe3O4, CeO2, and gold nanoparticles, exhibit enzyme-like activities (e.g., oxidase, peroxidase).
Current nanozymes often lack substrate specificity, limiting their practical applications.
Molecularly imprinted polymers (MIPs) can create specific binding sites to enhance selectivity.

Purpose of the Study:

To describe the preparation protocol for MIP-coated Fe3O4 peroxidase-mimicking nanozymes.
To detail critical procedures for successful MIP-nanozyme synthesis.
To facilitate the application of enhanced nanozymes in various fields.

Main Methods:

Coating Fe3O4 nanoparticles with molecularly imprinted polymers (MIPs).
Utilizing MIPs to create specific substrate binding pockets.
Developing a detailed protocol for MIP-Fe3O4 nanozyme preparation.

Main Results:

MIP coating significantly enhances nanozyme selectivity, up to 100-fold.
MIPs enrich specific substrates, enabling selective oxidation.
Non-imprinted substrates are sterically hindered from accessing the catalytic core.

Conclusions:

MIP-coated nanozymes offer a strategy to overcome the specificity limitations of traditional nanozymes.
The described protocol facilitates the development of highly selective nanozymes.
MIP-nanozymes show promise for advanced analytical, biomedical, and environmental applications.