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Updated: Oct 23, 2025

Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines
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Anticancer Effect of ERM210 on Liver Cancer Cells Through ROS/Mitochondria-dependent Apoptosis Signaling Pathways

Jaihyung Lee1, Yi-Xi Gong2, Dan-Ping Xie2

  • 1Epigenetics Drug Discovery Center, Haeam Convalescence Hospital, Gyeonggi, Republic of Korea.

In Vivo (Athens, Greece)
|August 19, 2021
PubMed
Abstract

Insights

ERM210, a Korean traditional medicine, effectively combats liver cancer cells by inducing apoptosis and inhibiting migration. This study reveals its mechanism involves reactive oxygen species (ROS) and mitochondria-dependent pathways.

Area of Science:

  • Traditional East Asian Medicine
  • Cancer Biology
  • Molecular Pharmacology

Background:

  • Asian traditional medicines possess known antitumor properties.
  • ERM210 is a Korean traditional medicine composed of nine medicinal plants.
  • Its potential in treating liver cancer warrants investigation.

Purpose of the Study:

  • To investigate the pro-apoptotic effects of ERM210 on HepG2 liver cancer cells.
  • To elucidate the molecular mechanisms underlying ERM210's action.
  • To explore ERM210 as a potential therapeutic agent for liver cancer.

Main Methods:

  • Cytotoxicity assessed via MTT assay.
  • Cell viability and migration studied using wound-healing assays.
  • Apoptosis and signaling pathways analyzed by flow cytometry and Western blotting.

Main Results:

  • ERM210 significantly reduced HepG2 cell viability and enhanced apoptosis.
  • Apoptosis was mediated by up-regulation of caspases 3, 7, 9 and BAX, and down-regulation of BCL2.
  • ERM210 increased reactive oxygen species (ROS) and inhibited cell migration, effects reversed by N-acetylcysteine.

Conclusions:

  • ERM210 exhibits anticancer effects on HepG2 cells through ROS/mitochondria-dependent apoptosis.
  • ERM210 modulates key apoptosis regulators and signaling pathways.
  • Findings support ERM210's potential for clinical application in liver cancer treatment.
Keywords:
AnticancerERM210apoptosismitochondriareactive oxygen species

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