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The rate theory of chromatography provides quantitative insight into the shapes and widths of elution bands. These bands are based on the random-walk mechanism governing molecular migration within a column. The Gaussian profile of chromatographic bands arises from the cumulative effect of random molecular motions as they progress through the column.
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Optimal data, added value

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  • 1Department of Cardiothoracic Surgery, Erasmus MC, Rotterdam, Netherlands.

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