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Protein Kinases and Phosphatases

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...

Protein Kinases and Phosphatases

Protein Kinases and Phosphatases

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Updated: Oct 23, 2025

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

ProfKin: A comprehensive web server for structure-based kinase profiling.

Zihao Shen¹, Yu-Hang Yan², Shuo Yang¹

¹Shanghai Key Laboratory of New Drug Design, East China University of Science and Technology, Shanghai, 200237, China.

European Journal of Medicinal Chemistry

|August 19, 2021

Summary

This summary is machine-generated.

ProfKin is a new web server for structure-based kinase profiling. It helps drug discovery by predicting kinase-ligand interactions using the KinLigDB database.

Keywords:

Interaction fingerprints Kinase Kinase inhibitor selectivity Kinase profiling Web server

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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

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Published on: August 2, 2015

Area of Science:

Biochemistry
Structural Biology
Pharmacology

Background:

Protein kinases are crucial in cell signaling and are key drug targets.
Kinase inhibitor selectivity is vital for drug discovery due to structural similarities among kinases.

Purpose of the Study:

To introduce ProfKin, a web server for structure-based kinase profiling.
To provide a comprehensive resource for analyzing kinase-ligand interactions and aiding drug discovery.

Main Methods:

Utilizes the KinLigDB database containing 4219 kinase-ligand complex structures.
Employs a method for predicting binding modes and prioritizing them using interaction fingerprint analysis.
Offers customizable database filtering by kinase subfamily and binding site type.

Main Results:

Provides 3D structures for 297 human kinases and associated binding information.
Ranks kinases based on a comprehensive index derived from predicted binding modes.
Enables visual inspection of predicted binding poses against reference modes.

Conclusions:

ProfKin facilitates structure-based kinase profiling for drug discovery.
The server enhances the understanding of kinase-ligand interactions and selectivity.
It offers a valuable tool for researchers investigating kinase inhibitors.