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Updated: Oct 23, 2025

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
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Using single-cell sequencing technology to detect circulating tumor cells in solid tumors.

Jiasheng Xu1,2,3, Kaili Liao4, Xi Yang5

  • 1Department of Gastroenterology, Huzhou Central Hospital, Affiliated Huzhou Hospital Zhejiang University School of Medicine, Huzhou, Zhejiang, China.

Molecular Cancer
|August 20, 2021
PubMed
Summary
This summary is machine-generated.

Single-cell sequencing of circulating tumor cells offers a novel approach to overcome tumor heterogeneity. This method analyzes individual cells to reveal genomic insights crucial for understanding tumor biology and guiding treatment.

Keywords:
Circulating tumor cellsResearch progressSingle-cell sequencingTumor heterogeneity

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Area of Science:

  • Oncology
  • Genomics
  • Molecular Biology

Background:

  • Circulating tumor cells (CTCs) possess high vitality and metastatic potential, shedding from primary or metastatic tumors into peripheral blood.
  • Tumor heterogeneity poses challenges for traditional high-throughput sequencing of tumor tissues, obscuring characteristics of low-abundance tumor stem cells.

Purpose of the Study:

  • To describe the identification, biological characteristics, and genome-wide variations of CTCs using single-cell sequencing.
  • To explore the application of single-cell sequencing of CTCs in tumor typing, metastasis analysis, progression detection, and adjuvant therapy.

Main Methods:

  • Single-cell sequencing of CTCs from peripheral blood.
  • Comparative analysis of single-cell genomes, transcriptomes, and epigenetic groups.
  • Comparison between CTCs, primary tumors, metastatic tumors, and metastatic lymph nodes.

Main Results:

  • Single-cell sequencing of CTCs effectively bypasses the limitations imposed by tumor heterogeneity.
  • This approach provides a new perspective for understanding tumor biological processes.
  • Identified genomic variations within CTCs.

Conclusions:

  • Single-cell sequencing of CTCs is a powerful tool for dissecting tumor biology and heterogeneity.
  • It offers significant potential for advancing cancer diagnostics and therapeutics, including personalized medicine.