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Related Concept Videos

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

6.1K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
6.1K

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Related Experiment Video

Updated: Oct 23, 2025

Miniaturized Sample Preparation for Transmission Electron Microscopy
09:04

Miniaturized Sample Preparation for Transmission Electron Microscopy

Published on: July 27, 2018

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A simple pressure-assisted method for MicroED specimen preparation.

Jingjing Zhao1, Hongyi Xu2, Hugo Lebrette3

  • 1Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden.

Nature Communications
|August 20, 2021
PubMed
Summary
This summary is machine-generated.

A new Preassis method simplifies specimen preparation for Micro-crystal electron diffraction (MicroED), overcoming a key hurdle. This technique allows controlled ice thickness for better macromolecular structure determination from tiny crystals.

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Area of Science:

  • Structural Biology
  • Biophysics
  • Materials Science

Background:

  • Micro-crystal electron diffraction (MicroED) is crucial for determining structures of small macromolecular crystals.
  • Specimen preparation is a significant challenge limiting MicroED's widespread use.

Purpose of the Study:

  • To develop a simple and universal method for MicroED specimen preparation.
  • To address the bottleneck of preparing suitable specimens for MicroED analysis.

Main Methods:

  • Introduced 'Preassis,' a method using an EM grid and pressure to remove excess liquid from specimens.
  • Controlled ice thickness by adjusting pressure and EM grid carbon hole sizes.
  • Tested the method with diverse protein crystals and buffer conditions.

Main Results:

  • Preassis enables controlled ice thickness for MicroED specimen preparation.
  • The method successfully processed various protein crystals, including viscous buffers and low concentrations.
  • Demonstrated a simple and effective approach for preparing MicroED specimens.

Conclusions:

  • Preassis is a simple, universal method for MicroED specimen preparation.
  • This technique can significantly expand the application scope of MicroED.
  • Facilitates structure determination of challenging macromolecular samples.