Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Heart Failure II: Pathophysiology01:29

Heart Failure II: Pathophysiology

108
Systolic Heart Failure and Compensatory MechanismsSystolic heart failure (also termed HFrEF, Heart Failure with Reduced Ejection Fraction) is the most prevalent type of heart filure. It results in a decreased volume of blood being pumped from the ventricle. The aortic arch and carotid sinuses have baroreceptors that detect reduced blood pressure, triggering the sympathetic nervous system (SNS) to release epinephrine and norepinephrine. Initially, this response aims to boost heart rate and...
108
Pathophysiology of Heart Failure01:17

Pathophysiology of Heart Failure

2.0K
Heart failure (HF) is a progressive syndrome involving ventricles that leads to inadequate cardiac output. It can be classified based on location and output or ejection fraction. Ejection fraction (EF) is an essential measurement in the diagnosis and surveillance of HF. Reduced EF corresponds to systolic heart failure (HFrEF). However, HF with preserved ejection fraction (HFpEF) is becoming increasingly prevalent. Also known as diastolic HF, this form of HF is related to aging. The...
2.0K
Inflammation01:38

Inflammation

56.5K
Overview
56.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of the MonCœur smartphone application on self-care and quality of life in heart failure: The APPLIMONCOEUR randomized trial.

Archives of cardiovascular diseases·2026
Same author

Mapping neuro-vascular unit communications reveals distinct angiogenic programs across developing mouse brain regions.

Nature communications·2026
Same author

Intrinsic capacity evolution during aging in mouse and fish: Longitudinal perspectives from a narrative review.

Ageing research reviews·2026
Same author

AI-guided integration of aging-related functional assessment to predict nonresponse to cardiac rehabilitation in heart failure.

The journal of nutrition, health & aging·2026
Same author

Association of advanced coronary artery calcification assessed by coronary artery calcium scoring with lipoprotein (a) and carotid atherosclerosis in asymptomatic patients.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2026
Same author

The spatiotemporal dynamics of postnatal vascularization in the mouse brain.

Cell·2026

Related Experiment Video

Updated: Oct 23, 2025

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
07:25

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Published on: December 17, 2019

8.3K

Cardiac macrophage subsets differentially regulate lymphatic network remodeling during pressure overload.

Mathilde Bizou1,2, Romain Itier1,3,2, Mina Majdoubi1,2

  • 1I2MC, Toulouse University, Inserm, Université Paul Sabatier, Toulouse, France.

Scientific Reports
|August 20, 2021
PubMed
Summary

Cardiac macrophages play a key role in heart failure progression. Specific macrophage subsets regulate lymphatic vessel remodeling, impacting heart function during pathological hypertrophy.

More Related Videos

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node
08:43

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

Published on: May 7, 2021

4.7K
Isolation and Identification of Extravascular Immune Cells of the Heart
08:24

Isolation and Identification of Extravascular Immune Cells of the Heart

Published on: August 23, 2018

13.4K

Related Experiment Videos

Last Updated: Oct 23, 2025

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
07:25

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Published on: December 17, 2019

8.3K
Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node
08:43

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

Published on: May 7, 2021

4.7K
Isolation and Identification of Extravascular Immune Cells of the Heart
08:24

Isolation and Identification of Extravascular Immune Cells of the Heart

Published on: August 23, 2018

13.4K

Area of Science:

  • Cardiovascular Biology
  • Immunology
  • Lymphatic System Research

Background:

  • The cardiac lymphatic network regulates interstitial fluid and immune cell movement.
  • Heart hypertrophy and failure lead to cardiac lymphatic remodeling and reduced efficiency.

Purpose of the Study:

  • To investigate the role of cardiac macrophage subpopulations in regulating cardiac lymphatic remodeling during pressure overload.
  • To identify specific macrophage markers and functions involved in pathological cardiac hypertrophy.

Main Methods:

  • Induction of heart hypertrophy and failure via transverse aortic constriction in mice.
  • Analysis of cardiac lymphatic vessels and macrophage populations (LYVE-1 positive/negative).
  • Assessment of lymphangiogenesis and gene expression (MMP12, CCR2).
  • Treatment with a CCR2 antagonist to evaluate its effect on macrophage subsets and lymphatic remodeling.

Main Results:

  • Cardiac lymphatic remodeling and reduced efficiency were observed during heart failure.
  • A subset of LYVE-1 positive cardiac macrophages promoted lymphangiogenesis.
  • LYVE-1 negative macrophages expressed MMP12, contributing to lymphatic remodeling.
  • CCR2 antagonist treatment preserved the lymphatic network by altering macrophage proportions.

Conclusions:

  • Distinct cardiac macrophage subpopulations have differential roles in regulating cardiac lymphatic function during pathological hypertrophy.
  • Macrophage-mediated lymphatic remodeling is a key mechanism in heart failure progression.
  • Targeting CCR2-mediated pathways may offer therapeutic strategies for heart failure.