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The peppermint breath test benchmark for PTR-MS and SIFT-MS.

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  • 1Department of Molecular and Laser Physics, Radboud University, Nijmegen, The Netherlands.

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Standardizing breath analysis is crucial. The Peppermint Experiment used proton transfer reaction mass spectrometry (PTR-MS) and selected ion flow tube mass spectrometry (SIFT-MS) to establish benchmark values for peppermint volatiles in exhaled breath.

Keywords:
PTR-MSSIFT-MSbenchmarkbreathomicspeppermintpharmacokineticsstandardization

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Area of Science:

  • * Breath analysis and metabolomics
  • * Analytical chemistry and mass spectrometry

Background:

  • * Lack of standardization in breath research hinders comparability across studies.
  • * The Peppermint Experiment was designed to address standardization challenges.
  • * It uses a standardized intervention and defined protocol for analyzing exhaled breath volatiles.

Purpose of the Study:

  • * To explore disparities in breath research across different analytical platforms.
  • * To provide benchmark values for comparison in breath analysis.
  • * To investigate the breath washout profiles of peppermint volatiles.

Main Methods:

  • * Ingestion of encapsulated peppermint oil followed by targeted analysis of exhaled breath.
  • * Utilized proton transfer reaction mass spectrometry (PTR-MS) and selected ion flow tube mass spectrometry (SIFT-MS).
  • * Analyzed key peppermint volatiles including limonene, pinenes, 1,8-cineole, menthol, menthone, and menthofuran in 65 individuals.

Main Results:

  • * Collected breath washout profiles for peppermint volatiles across five PTR-MS and two SIFT-MS instruments.
  • * Calculated benchmark washout values, e.g., PTR-MS: 353 min (monoterpenes/1,8-cineole), 173 min (menthol); SIFT-MS: 228 min (monoterpenes).
  • * Observed significant inter- and intra-dataset variations, indicating a substantial role for biological variability.

Conclusions:

  • * Biological variability significantly influences the absorption, metabolism, and excretion of compounds via breath.
  • * The observed variability may outweigh the impact of sampling and analytical procedures.
  • * Further research is recommended to fully elucidate the effects of sampling and analytical factors on breath volatile profiles.