Fully integrated sampler and dilutor in an electrochemical paper-based device for glucose sensing

Area of Science:

• Electrochemistry
• Biosensing
• Analytical Chemistry

Background:

• Traditional analytical methods often involve multiple complex steps.
• There is a need for integrated, portable devices for decentralized analysis.

Purpose of the Study:

• To design and demonstrate an all-in-one electroanalytical platform for biosensing applications.
• To integrate sampling, dilution, and electrochemical detection into a single device.

Main Methods:

• Development of a paper-based electrochemical cell with integrated sampling and dilution pads.
• Utilized glass-fiber pads for sample collection and dilution.
• Incorporated a paper-based working electrode and low-cost connector headers for reference and counter electrodes.
• Developed an enzymatic glucose biosensor using ferrocyanide as a mediator.
• Demonstrated sucrose detection by incorporating invertase to hydrolyze sucrose into glucose and fructose.

Main Results:

• Successfully designed and validated an integrated electroanalytical platform on paper.
• Achieved accurate glucose determination in real food samples (cola, orange juice).
• Demonstrated proof-of-concept for sucrose detection via enzymatic hydrolysis and subsequent glucose measurement.

Conclusions:

• The developed "lab-on-paper" platform offers a cost-effective and time-saving approach for decentralized analysis.
• This integrated system enhances accuracy and accessibility for biosensing applications.
• The platform shows significant potential for developing advanced, portable analytical devices.