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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Oct 23, 2025

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

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CRISPR-Cas based virus detection: Recent advances and perspectives.

Lijuan Yin1, Shuli Man1, Shengying Ye2

  • 1State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China.

Biosensors & Bioelectronics
|August 21, 2021
PubMed
Summary

CRISPR-Cas systems offer a revolutionary approach to detecting viruses like SARS-CoV-2. These advanced biosensing platforms provide rapid, sensitive, and specific identification crucial for disease control.

Keywords:
CRISPR-CasCRISPR-based detectionCollateral cleavage (trans-cleavage)Point-of-care testingSingle nucleotide polymorphism detectionVirus detection

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Infectious Diseases

Background:

  • Viral infections, such as COVID-19 caused by SARS-CoV-2, pose significant global health threats.
  • Current viral detection methods have limitations, necessitating the development of novel, field-deployable biosensing platforms.
  • CRISPR-Cas systems offer enhanced sensitivity, specificity, and programmability for molecular diagnostics.

Purpose of the Study:

  • To review recent advancements in virus detection utilizing CRISPR-Cas systems, particularly CRISPR-Cas12a and CRISPR-Cas13a.
  • To highlight the significance and advantages of CRISPR-Cas-based biosensing for viral identification.
  • To discuss challenges and future perspectives in this rapidly expanding field.

Main Methods:

  • Review of current literature on CRISPR-Cas systems (Cas12a, Cas13a) applied to virus detection.
  • Analysis of the properties and applications of CRISPR-Cas effectors in molecular diagnostics.
  • Discussion of the advantages and limitations of CRISPR-Cas based biosensing technologies.

Main Results:

  • CRISPR-Cas systems, especially Cas12a and Cas13a, are being repurposed for highly sensitive and specific virus detection.
  • These systems enable rapid, field-deployable diagnostic tools, revolutionizing viral identification.
  • Recent advances showcase the potential of CRISPR-Cas technology in various virus detection scenarios.

Conclusions:

  • CRISPR-Cas based biosensing represents a significant advancement in molecular diagnostics for viral pathogens.
  • The sensitivity, specificity, and programmability of CRISPR-Cas systems offer a promising alternative to existing detection methods.
  • Further development and application of CRISPR-Cas technology hold great potential for global health surveillance and disease management.