[Reliability study on establishing a rat tuberculosis wound model using Bacillus Calmette-Guérin].

Area of Science:

• Microbiology
• Infectious Diseases
• Animal Models

Background:

• Tuberculosis remains a significant global health challenge.
• Reliable animal models are crucial for studying tuberculosis pathogenesis and developing new treatments.

Purpose of the Study:

• To assess the reliability and stability of a rat model for tuberculous wounds.
• To establish a reproducible experimental system for tuberculosis research.

Main Methods:

• Sprague-Dawley rats were divided into control and infection groups.
• Tuberculous wounds were induced by subcutaneous injection of Bacillus Calmette-Guérin (BCG).
• Histopathological analysis (hematoxylin-eosin, acid-fast staining) and gross observation were performed at multiple time points.

Main Results:

• Gradual development of tuberculous wound lesions was observed in the BCG-infected group.
• Histopathology revealed progressive granuloma formation, necrosis, and inflammatory cell infiltration.
• Acid-fast staining confirmed the presence of a large number of bacteria in lesion tissues by day 43.

Conclusions:

• The rat model established with BCG is stable and reliable for experimental use.
• This model accurately reflects key pathological features of tuberculosis.
• The model is suitable for further investigations into tuberculosis wound healing and treatment strategies.