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A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
Published on: January 4, 2017
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Extracellular matrix extraction techniques and applications in biomedical engineering
Kirthanashri S Vasanthan1, Varadharajan Srinivasan2, Deepti Pandita3
1Amity Institute of Molecular Medicine & Stem cell Research (AIMMSCR), Amity University Uttar Pradesh, Sector-125, Noida, 201313, India.
Regenerative Medicine
|August 24, 2021
Summary
Tissue engineering uses decellularized extracellular matrix (dECM) scaffolds for tissue repair. This review details dECM methods and biomedical applications for organ regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Tissue engineering aims to regenerate damaged tissues and organs.
- The extracellular matrix (ECM) is crucial for scaffold development and cell proliferation.
- Decellularized ECM (dECM) retains native biomolecules while removing cells.
Purpose of the Study:
- To review decellularization techniques for various animal organs.
- To explore biomedical applications of dECM.
- To highlight dECM's role in creating suitable microenvironments for organ regeneration.
Main Methods:
- Detailed methodologies of diverse decellularization techniques are discussed.
- Optimization of decellularization methods is presented.
- Assessment of dECM properties including microarchitecture, protein content, and biocompatibility is detailed.
Main Results:
- Decellularization techniques preserve essential biomolecules within the ECM.
- Optimized dECM provides an in vivo-mimicking microenvironment for in vitro regeneration.
- dECM demonstrates biocompatibility and supports cell proliferation.
Conclusions:
- dECM is a promising biomaterial for tissue engineering and regenerative medicine.
- dECM scaffolds can be utilized for organ regeneration, drug delivery, and tissue repair.
- Further research into dECM processing and applications holds significant potential.
Keywords:
3D bioprintingbioinksdecellularizationdecellularized extracellular matrixdrug-deliverymicroparticlesnanofiberspreserving ECMMore Related Videos
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