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Method for high-throughput antifungal activity screening of bacterial strain libraries
Jeffrey D Palumbo1, Teresa L O'Keeffe1
1Foodborne Toxin Detection and Prevention Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Albany, CA, USA.
Journal of Microbiological Methods
|August 24, 2021
Abstract:
Our laboratory developed a high-throughput screen for antifungal phenotypes in which bacterial libraries in 96-well format are transferred to agar pour plates inoculated with spores of the target fungus. This method is an improvement over bacterial/fungal coculture plate methods that measure antifungal activity as inhibition of radial fungal growth.
