Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

3.7K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.7K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

10.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
10.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activatable smart contrast agents for photoacoustic imaging.

Smart molecules : open access·2026
Same author

Monolithic 3D-Integrated All-Solid Ion-Gated Carbon Nanotube Transistors With Tunable Ionic Conductance for Multi-Timescale Reservoir Computing.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Multiscale photoacoustic imaging of stroke in preclinical models and future directions toward clinical translation [Invited].

Biomedical optics express·2026
Same author

Pulse Repetition Frequency Tuned Low-Intensity Focused Ultrasound Neuromodulation for Tibial Nerve Targeted Bladder Function Modulation.

IEEE transactions on bio-medical engineering·2026
Same author

Introduction to the feature issue Photoacoustic Imaging and Sensing: Beyond Fundamentals to Translation.

Biomedical optics express·2026
Same author

Fully integrated photoacoustic microscopy for multi-scale and longitudinal imaging in translational biomedical applications.

Biomedical optics express·2026
Same journal

Peripheral B-cell receptor repertoire predicts immune-related adverse events following immune checkpoint inhibitor therapy in advanced renal cell carcinoma.

Scientific reports·2026
Same journal

Effects of black soldier fly (Hermetia illucens L.) larvae zoocompost on the mineral element content of blue honeysuckle berries.

Scientific reports·2026
Same journal

Investigation on absorption refrigeration performance of R1243zf with imidazolium ionic liquid as the working pairs.

Scientific reports·2026
Same journal

DeepTriage-CN: integrating clinical text with vital signs for emergency department admission prediction in an aging population.

Scientific reports·2026
Same journal

Gold nanoparticles as dual-action antiviral agents: disruption of SARS-CoV-2 viral envelopes and RNA integrity.

Scientific reports·2026
Same journal

Comparison of capillary microsampling and venous blood for multi-pathogen serosurveillance.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Oct 23, 2025

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

11.9K

Dual-pulse photoactivated atomic force microscopy.

Byullee Park1, Seunghyun Lee1, Jimin Kwon1

  • 1Departments of Electrical Engineering, Medical Device Innovation Center, Convergence IT Engineering, Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.

Scientific Reports
|August 25, 2021
PubMed
Summary
This summary is machine-generated.

Dual-pulse photoactivated atomic force microscopy (DP-pAFM) enhances nanoscale imaging by using two laser pulses. This novel method maps optical structures and visualizes critical nanoscale cracks in semiconductors without damaging the sample.

More Related Videos

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
08:59

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

940
Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

9.0K

Related Experiment Videos

Last Updated: Oct 23, 2025

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

11.9K
High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
08:59

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

940
Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
06:45

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

9.0K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Physics

Background:

  • Photoactivated atomic force microscopy (pAFM) is crucial for nanoscale structure analysis.
  • Increasing laser power in pAFM improves signal-to-noise but risks sample and tip damage.
  • Nanoscale cracks in organic semiconductors significantly impede device performance.

Purpose of the Study:

  • To develop a novel pAFM technique that enhances signal without causing damage.
  • To effectively map optical structures and defects in small-molecule semiconductor films.
  • To visualize critical nanoscale cracks in organic semiconductors.

Main Methods:

  • Implementation of a dual-pulse pAFM (DP-pAFM) technique.
  • Utilizing two time-delayed laser pulses to control heating and mechanical vibration.
  • Analyzing optical structures and nanoscale cracks in semiconductor films.

Main Results:

  • DP-pAFM successfully mapped optical structures of small-molecule semiconductor films.
  • The technique clearly visualized nanoscale cracks, a key issue in organic semiconductors.
  • DP-pAFM demonstrated improved signal-to-noise without compromising sample integrity.

Conclusions:

  • DP-pAFM offers a damage-free approach to boost pAFM signal-to-noise ratio.
  • This method provides a new optical pathway for studying nanoscale phenomena.
  • DP-pAFM is a promising tool for analyzing complex materials and defects.