Related Experiment Video
Updated: Oct 23, 2025
06:38
Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
Published on: October 12, 2018
19.2K
Comprehensive in situ mapping of human cortical transcriptomic cell types.
Christoffer Mattsson Langseth1, Daniel Gyllborg1, Jeremy A Miller2
1Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden.
Communications Biology
|August 25, 2021
Summary
Researchers created the first spatial atlas of human cortical cells, mapping 59,816 cells into 75 subtypes. This provides a foundational resource for understanding brain function and disease at a cellular level.
Area of Science:
- Neuroscience
- Genomics
- Cell Biology
Background:
- Understanding human brain function requires spatially resolving cellular architecture.
- Existing methods lack comprehensive spatial mapping of cortical cell types.
Purpose of the Study:
- To create the first spatial atlas of human cortical cells.
- To map cell types, abundances, and genetic signatures in their native positions.
- To provide a reference for future brain tissue and disease studies.
Main Methods:
- Combined in situ sequencing gene expression data with single-nucleus RNA-sequencing cell type definitions.
- Employed probabilistic cell typing to spatially map cells in human cortex sections.
- Mapped and classified 59,816 cells into 75 previously defined subtypes.
Main Results:
- Generated a comprehensive spatial atlas of human cortical cells.
- Detailed the precise within- and across-layer distributions of all mapped cell types.
- Identified cell abundances and genetic signatures within the human cortex.
Conclusions:
- The developed spatial atlas is a key resource for the cell atlas community.
- Provides a reference for human brain tissue research and disease applications at the cell type level.
- Enables deeper investigation into brain function through spatial cell distribution.
